citation situation difficile

Front Microbiol. Rev. Clostridium difficile infection appears particularly after antibiotic chemotherapy or prolonged periods of hospitalization, which causes disruption and dysbiosis of the endogenous intestinal microbiota and facilitates the proliferation of toxigenic C. difficile in the gut. Fecal microbiota transplantation for primary Clostridium difficile infection. Fecal-oral transmission from other patients or animals, which represent potential reservoirs of C. difficile, also plays an important role in CDI epidemiology (Rupnik, 2007). Clostridium difficile infection (CDI) rates in the United States have tripled from 2000 to 2005, and disease morbidity and mortality have increased, particularly among elderly persons. J. Med. Species and genus level resolution analysis of gut microbiota in Clostridium difficile patients following fecal microbiota transplantation. In addition, an elevated abundance of Akkermansia may be a predictive marker for the presence of a CDI. Cell. Microbiol. PDF | Women's access to land has been at the heart of the news for three decades. This study was conducted at the Hospital Universitario “Rio Hortega” (Valladolid, Castilla y León, Spain) in accordance with the recommendations of the Ethical Clinical Research Committee (CEIC) of the western health area of Valladolid. (2012). Environ. Wilson and Perini (1988) and latter other authors, demonstrated that other microorganisms compete more efficiently than C. difficile for monomeric glucose, N-acetylglucosamine, and sialic acids found in colonic contents, being the reason why C. difficile is not a predominant intestinal bacterium. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). (2018). Here, we characterized the gut microbiota from the feces of 57 patients with diarrhea from nosocomial and community-acquired CDI. coli, identification of virulence genes ofE. Relative abundance of Peptostreptococcaceae family within the order Clostridiales was comparatively low, 1.56% overall, and C. difficile was the most abundant species within both the order Clostridiales (Figure 4) and the family Peptostreptococcaceae (Figure 5). Here are specific examples of how it works in the three major citation styles: APA Style. Microbiol., 14 January 2019 JColorGrid: software for the visualization of biological measurements. Citations situation difficile Sélection de 3 citations et proverbes sur le thème situation difficile Découvrez un dicton, une parole, un bon mot, un proverbe, une citation ou phrase situation difficile issus de livres, discours ou entretiens. Impact Factor 4.235 | CiteScore 6.4More on impact ›, The Deadly Secrets of C. Difficile - Insights into Host-Pathogen Interaction La plupart des femmes ont toujours assez d'esprit pour se tirer d'une situation difficile. Heat map showing the distribution and relative abundance of members of the Peptostreptococcaceae family grouped (in rows) within the different samples (in columns). Les plus belles citations. (2016). A total of 7,474,887 reads survived the quality filtering process (108,239 ± 53,034 reads/sample). Patients in cluster B had had a history of less aggressive antibiotherapy, less severe diarrhea, and only a small incidence of recurrence. In contrast, the diarrhea was less severe and the patients who provided samples in which Bacteroidaceae was the most abundant family (mainly Cluster B) recovered easily. ISME J. However, co-infection with other pathogenic agents often plays an important role in the development of diarrhea and must be considered when prescribing antibiotic treatment. C. difficile. Definition of difficult in the Definitions.net dictionary. Check out our other citation guides on MLA 8 and Harvard referencing. The purpose of this policy statement is to provide the pediatrician with updated information and recommendations about C difficile infections affecting pediatric patients. The previous history of antibiotherapy is also highly relevant; the trigger may be dysbiosis caused by prior antibiotic treatment, even in cases in which the gastrointestinal process is caused by C. difficile and there is robust evidence for CDI. Thus, a common sign of antibiotic exposure is the reduction or absence of Lachnospiraceae in the gut, creating an environmental niche for opportunistic CDI (Song et al., 2013). All subjects gave written informed consent in accordance with the Declaration of Helsinki. The epidemiology of Clostridium difficile-associated diarrhea (CDAD) is not well defined in nonepidemic situations because precise biotyping techniques have only recently become available. The Gram-positive, spore-forming anaerobe, Clostridioides difficile (formerly Clostridium difficile and Peptoclostridium difficile; Yutin and Galperin, 2013) is an asymptomatic component of the healthy intestinal microbiota of approximately 2–7% of healthy human adults and up to 70% of healthy newborns (McFarland et al., 1989; Lees et al., 2016). Dis. QIIME allows analysis of high-throughput community sequencing data. Culture-based studies suggest that all healthy adults share most of the same gut bacterial species, whereas culture-independent sequencing studies have revealed vast microbial diversity (more than 1,000 species), that varies highly over time and among the population. Sangster et al. The individuals in this study clearly belonged to enterotype 1, with a high abundance of Bacteroides (31.01% overall). The Citation Latitude aircraft flight deck, centered around Garmin G5000 avionics, is designed to provide pilots with the comfort and information they need for a reliable and efficient flight. Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Here, we characterized the gut microbiota from the feces of 57 patients with diarrhea from nosocomial or community-acquired CDI. DADA2: high-resolution sample inference from Illumina amplicon data. 14, 1208–1219. Pilots can take command of the aircraft’s robust navigation, traffic, surveillance and communications systems with ease. We analyzed only samples from patients with diarrhea and a positive diagnosis for C. difficile. MH and NQ performed the bioinformatics analysis and designed the figures. (2016). Leffler, D. A., and Lamont, J. T. (2015). doi: 10.1111/1462-2920.12173, Keywords: CDI, Clostridioides difficile, diarrhea, microbiota, bacterial, 16S rRNA, Citation: Hernández M, de Frutos M, Rodríguez-Lázaro D, López-Urrutia L, Quijada NM and Eiros JM (2019) Fecal Microbiota of Toxigenic Clostridioides difficile-Associated Diarrhea. Sources that have no known author or editor should be cited by title. However, the percentage of Bacteroides was lower among the most affected patients and in those with the worst evolution; a reduction in the abundance of this family to below 50% could be considered to be a marker for worsening of the clinical prognosis. Figure 3. Clostridium difficile (C. difficile) is a Gram-positive, spore-forming, anaerobic bacillus, which is widely distributed in the intestinal tract of humans and animals and in the environment. In addition, the PCoA plot showed the points to be more widely dispersed in cluster A than in cluster B (Figure 2). Received: 27 September 2018; Accepted: 21 December 2018;Published: 14 January 2019. Other relevant factors associated with the emergence of CDI have been the introduction of C. difficile strains resistant to multiple antibiotics, including metronidazole, as well as genomic plasticity and the potential to transfer resistance genes, as approximately 11% of the C. difficile genome consists of mobile genetic elements (Sebaihia et al., 2006). We performed an ecological analysis by high-throughput sequencing of the V3-V4 region of 16S rRNA amplicons and evaluated the association of the various ecological profiles with CDI risk factors. Anonymous works--Unknown authorship. *Correspondence: Jose María Eiros, jmeirosbouza@gmail.com, Front. No use, distribution or reproduction is permitted which does not comply with these terms. Nat. Nat. doi: 10.1056/NEJMc1803103, Katoh, K., and Standley, D. M. (2013). Most are obligate anaerobes, indicating severe changes in the redox potential that can produce gut bacterial dysbiosis. Dis. Sixty-five fecal samples of the 57 patients with diarrhea enrolled in this study were studied. {{ U }}) while signing a reply, thx 12:01, 6 October 2013 (UTC) Alpha and beta diversity analysis were performed using the diversity3 and emperor (Vazquez-Baeza et al., 2013) plugins. Genet. 28, 721–741. The characterization was based on phylogenetic typing ofE. Evol. Diversity and evolution in the genome of Clostridium difficile. We detected C. difficile in 73.33% of the samples of cluster A. Interestingly, there was a positive correlation between the increase in the abundance of Akkermansia and that of the Clostridioides genus, as it includes only Clostridioides difficile (Figure 6). A genomic update on clostridial phylogeny: Gram-negative spore formers and other misplaced clostridia. Alcalá, L., Reigadas, E., Marín, M., Martín, A., Catalán, P., and Bouza, E. (2015). J. Med. Together with Bacteroidaceae, these five bacterial families represented almost two thirds of the bacterial diversity. Prevotellaceae is a family that can enzymatically disrupt mucosal barrier function and tends to be more abundant in intestinal biopsy samples isolated from patients with inflammatory bowel disease (IBD) (Nagao-Kitamoto et al., 2016). In … (2014). The 7th edition was published in 2020 and is the most recent version. doi: 10.1093/molbev/mst010, Klindworth, A., Pruesse, E., Schweer, T., Peplies, J., Quast, C., Horn, M., et al. Clostridium difficile infection (CDI), especially hospital-acquired Clostridium difficile infection (HA-CDI), continues to be a public health problem and has aroused great concern worldwide for years. Nosocomial acquisition of Clostridium difficile infection. Although Lachnospiraceae are common inhabitants of the healthy human and mammalian gut microbiota (Lagier et al., 2012), they are highly sensitive to antimicrobial chemotherapy. Optimizing taxonomic classification of marker-gene amplicon sequences with qiime 2’s q2-feature-classifier plugin. Plotting was carried out in the R environment4, using ggplot2 (Wickham, 2016) and reshape2 (Wickham, 2007) packages. Several minority bacterial families (with an overall relative abundance below 5%), such as Enterococcaceae, Veillonellaceae, Rikenellaceae, Akkermansiaceae, Burkholderiaceae, Acidaminococcaceae, Prevotellaceae, Streptococcaceae, Barnesiellaceae, Peptostreptococcaceae, Staphylococcaceae, Marinifilaceae, and Fusobacteriaceae, were particularly abundant in some samples. 378, 2535–2536. Amplicon Sequence Variants (ASVs) were obtained instead and represent much higher taxonomic resolution than OTUs, as single-nucleotide differences over the sequenced gene region are taken into account (Callahan et al., 2017). Dis. This easy-to-use, comprehensive guide makes citing any source easy. Infect. (2014). 2, 10–14. Figure 5. Rupnik, M. (2007). The relative abundance of the 15 most abundant families in the fecal samples is shown in Figure 3, in which samples are segregated into clusters A and B from the PCoA. This study aimed to elucidate the clinical and epidemiological features of HA-CDI and the characteristics of C.difficile isolates in Chongqing, Southwest China. Citation: Ministry of Health and Ministry for Primary Industries. Impact of clinical awareness and diagnostic tests on the underdiagnosis of Clostridium difficile infection. CiteScore values are based on citation counts in a range of four years (e.g. Pour tous ceux et celles qui aiment les citations. How is Enfants En Situation Particulièrement Difficile (French) abbreviated? Reshaping data with the reshape package. C. difficile was identified in almost all samples and was detected in all but five diarrheic samples included in Cluster B (11.90% of the samples in cluster B), in which other components of the family Peptostreptococcaceae were found (not in sample MS0223). Google Scholar Citations lets you track citations to your publications over time. (2016) also reported an increase in the abundance of Akkermansia muciniphila in CDI patients, potentially linked to the capacity of Akkermansia to degrade mucin, which may provide a selective advantage in CDI. In southern Mauritania, men's control over land is a reality. Patients in cluster A were either immunosuppressed (transplant patients) or immunocompromised (cancer patients) and treated with severe antibiotic regimes, including broad spectrum antibiotics. C. difficile was absent from nine samples: asterisks indicate the negative samples of cluster A and black dots the negative samples of cluster B. ESPD stands for Enfants En Situation Particulièrement Difficile (French). Bacterial and fungal microbiota changes distinguish C. difficile infection from other forms of diarrhea: results of a prospective inpatient study. Enterobacteriaceae was the most abundant family in cluster A (22.66% overall, but ranged from 0% in sample MS1495 to 79.13% in sample MS1508), whereas the relative abundance of Lachnospiraceae was significantly lower (4.96% overall). 41:e1. 34, 1515–1525. Bacteroidaceae was the most abundant family overall (31.01%). For each stool sample, 220 mg of feces was homogenized and total DNA extracted using the QIAamp DNA Stool Mini Kit (Qiagen), according to manufacturer’s instructions. The immune system protects the host from pathogenic organisms (bacteria, viruses, fungi, parasites). View all Each citation style handles this situation a little bit differently! N. Engl. We observed a reduction in the abundance of Bacteroides not only in C. difficile associated diarrhea, but also when other pathogenic agents were more abundant. Antibiotherapy generally consisted of metronidazole and then vancomycin if the diarrhea persisted. 320, 204–210. Fecal Microbiota for Clostridium difficile Clostridium difficile is the leading cause of nosocomial infectious diarrhea. Front. If you get a photo citation, such as when a camera takes a photo of you in your car at a red light or at a railroad-grade crossing, you will get a notice in the mail about how to handle the ticket. Oral antibiotics such as metronidazole, vancomycin and fidaxomicin are therapies of choice for Clostridium difficile infection. 25:789. doi: 10.3389/fmicb.2016.00789, Schubert, A. M., Rogers, M. A. M., Ring, C., Mogle, J., Petrosino, J. P., Young, V. B., et al. Figure 1A summarizes the patient metadata; there were no significant differences between gender, 62% of the patients were ≥65 years old, 73.7% were healthcare-associated cases, and 19 patients (33.3%) died before August 2018. However, Firmicutes was more prevalent in 13 samples and Proteobacteria in three. (A) Distribution of the patients according to age (y, years and , average age), sex (female and male), and community-, healthcare in a nursing home-, and nosocomial-acquired diarrhea. 29K likes. Acquisition of C. difficile appears to be from the environment rather than maternal sources. First, a potential drawback of ecological studies of gut microbiota in pathological processes is the misdiagnosis and/or the misassumption of the role of C. difficile in the gastrointestinal process, as patients colonized by C. difficile can be found for whom the diarrhea is primarily caused by other pathogens, although C. difficile is present. ASV assigned to the family Peptostreptococcaceae were extracted from the feature table and used to construct a heatmap using JColorGrid (Joachimiak et al., 2006). Differences in the relative abundance of these bacterial families in clusters A and B are shown in Figure 3. Although one of the main alpha diversity indices, the Shannon index, was significantly higher in our study than that reported in other studies (Staley et al., 2018), the findings of our study are in accordance on that decreased diversity, as we observed reduced bacterial diversity; 3,477 different ASVs were identified with high variability between samples and 29 harbored less than 100 different ASVs. ESPD is defined as Enfants En Situation Particulièrement Difficile (French) rarely. PLoS One 8:e81330. Indeed, there was a significant difference in the distribution of Bacteroidaceae between cluster A and cluster B; the relative abundance of Bacteroidaceae was very low and even absent in many samples (5.57% overall, Figure 3A) in cluster A, whereas it was dominant in cluster B, with a relative abundance of 36.91% (Figure 3B). Citations difficulté - Découvrez 58 citations sur difficulté parmi les meilleurs ouvrages, livres et dictionnaires des citations d'auteurs français et étrangers. Among 500 isolates representing 59 PCR ribotypes a total of 35 high molecular weight types could be resolved. Here, we characterized the gut microbiota from the feces of 57 patients with diarrhea from nosocomial and community-acquired CDI. Microbiome 6:90. doi: 10.1186/s40168-018-0470-z, Callahan, B. J., McMurdie, P. J., and Holmes, S. P. (2017). MF, MH, and JE designed the study. J. Med. Clostridium difficile, a Gram-positive, spore-forming anaerobic bacillus, was first described in 1935, 1 but it was not associated with antibiotic-related diarrhea until the late 1970s. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EMPeror: a tool for visualizing high-throughput microbial community data. Biol. Many studies have characterized the baseline gut microbiota in healthy adults, in which 90% is composed of anaerobes. 35, 1047–1057. The average percentage of each genus among all samples is shown to the left of the name of the genus. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. However, the main alpha diversity indices, such as the Chao richness estimator, and the Shannon and Simpson diversity indices were 134.32 ± 80.6, 4.01 ± 1.25, and 0.86 ± 0.12, respectively, indicating that the observed reduced alpha diversity was true, and not a sequencing artifact. All patients were reported to be CDI positive as they were positive for GDH antigen and the gene encoding toxin B (tcdB), but negative for the C. difficile ribotype 027 test. That was true in our study for at least four patients who were infected by Klebsiella, Campylobacter, Staphylococcus, or Escherichia. 2:136. doi: 10.3389/fcimb.2012.00136, Lees, E. A., Miyajima, F., Pirmohamed, M., and Carrol, E. D. (2016). In the last decade, the frequency and severity of C. difficile infection has been increasing worldwide to become one of the most common hospital-acquired infections. We also observed a correlation between the presence of Akkermansia and Peptostreptococcaceae as previously reported (Sangster et al., 2016). Clostridium difficile toxins A and B: insights into pathogenic properties and extraintestinal effects. Enterotypes of the human gut microbiome. In these cases, co-infection cannot be ruled out, and the differences in the bacterial community profile should be considered with caution. C. difficile infection (CDI) is thus a toxin-mediated disease of the colon, with clinical symptoms that range from mild or self-limiting diarrhea to pseudomembranous colitis and life-threatening fulminant colitis (Leffler and Lamont, 2015; Smits et al., 2016). difficile, serotyping ofSalmonella spp., ribotyping and MLST ofC. The use of aggressive antibiotherapy with broad spectrum antibiotics may have reduced the abundance of Bacteroidaceae and Lachnospiraceae in cluster A, giving an advantage to Enterobacteriaceae and Enterococcaceae. This work received financial support of the Gerencia Regional de Salud of Junta de Castilla y León (Spanish Regional Government) (Grant Number GRS 1780/A/18). J. doi: 10.1093/cid/cis1209, Joachimiak, M. P., Weisman, J. L., and May, B. CDI is defined by the presence of symptoms (usually diarrhea) and either a stool test positive for C. difficile toxins or detection of toxigenic C. difficile, or colonoscopic or histopathologic findings revealing pseudomembranous colitis. Bacteroidaceae was the most abundant family along all samples, followed by Enterobacteriaceae. Sample multiplexing, library purification, and sequencing were carried out as described in the “16S Metagenomic Sequencing Library Preparation” guide by Illumina. Nucleic Acids Res. We observed lower bacterial diversity than that obtained using OTUs; between 32 (MS0218) and 352 (MS0138) different bacterial ASVs were identified in each fecal sample, significantly lower than the expected 1,000 OTUs that are estimated to exist in a healthy human gut. Yutin, N., and Galperin, M. Y. Clin. difficil… Meaning of difficult. Summarized below are recommendations intended to improve the diagnosis and management of Clostridium difficile infection (CDI) in adults and children. The rate of recurrence and mortality has been increasing since 2002, associated with severe infections produced by the emergence of the hyper-virulent ribotype 027 (ST1) strain. (2013). Relative abundance (%) of the 15 most abundant families found in the samples plotted in the A or B cluster of the PCoA: (A,B), respectively. Many studies of the human intestinal tract have reported few phyla to be present in the gut; Bacteroidetes and Firmicutes generally dominate, whereas Actinobacteria, Proteobacteria, and Verrucomicrobia, are generally minor constituents.

Casque Sans Fil Prise Jack, Ena Anciens élèves Célèbres, Chambre D'hote Frejus Plage, Take Out Orford, Canton Suisse En 3 Lettres, Horaire Train Vintimille Carnoles, Deuxième République Date, Citation Sur L'inexistence De Dieu, Vol Lyon - Nantes Hop,